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SMAD5 monoclonal antibody

货号: MB66501

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66501

Product Name :SMAD5 monoclonal antibody

Swiss-Prot :Q99717

Host :Mouse

Reactivity :Human

Applications :WB, IF/ICC, FC

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), FC (1/50 - 1/100)

Background :Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis . BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors . They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad9 (Smad8) at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes . MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1. Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region .

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of SMAD5 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ESC Pluripotency and Differentiation Signaling Pathway,TGFβ Smad signaling

Alternative Name :MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5

Immunogen :Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of SMAD5 expression in Hela (A), Jurkat (B), K562 (C) whole cell lysates.

Immunofluorescent analysis of SMAD5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.