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SMC1A monoclonal antibody

货号: MB66490

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66490

Product Name :SMC1A monoclonal antibody

Swiss-Prot :Q14683

Host :Mouse

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background :Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair. ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint . SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment . While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction . The serine residue at 360 of SMC1 is phosphorylated in an ATM/ATR-dependent manner in response to DNA damage . Phospho-SMC1 (Ser360) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser360 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of SMC1A protein.

BiowMW :~ 145 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8

Immunogen :Purified recombinant human SMC1A(C-term.) protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of SMC1A expression in Molt4 (A), U87MG (B), Hela (C) whole cell lysates.