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JMJD6 monoclonal antibody

货号: MB66474

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66474

Product Name :JMJD6 monoclonal antibody

Swiss-Prot :Q6NYC1

Host :Mouse

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background :Jumonji domain-containing protein 6 (JMJD6) is a bifunctional metalloenzyme belonging to the large family of JmjC domain-containing proteins. These proteins are ferrous iron- and 2-oxoglutarate-dependent enzymes (Fe2+/2OG) that catalyze hydroxylation and demethylation reactions on a wide variety of protein and nucleic acid substrates. JMJD6 is thought to act as both a lysyl-hydroxylase and an arginine demethylase, although the latter activity remains controversial. Specifically, JMJD6 has been shown to catalyze 5-hydroxylation of Lys15 and Lys276 residues on the protein U2AF2 / U2AF65 in vivo, affecting pre-mRNA splicing activity. It has also been reported that JMJD6 hydroxylates the Lys382 residue of p53, preventing acetylation and promoting association of p53 with MDMX, resulting in inhibition of p53 transcriptional activity. In addition to hydroxylase activity, JMJD6 also acts as an arginine demethylase by targeting histone H3 at Arg2 (H3R2me) and histone H4 at Arg3 (H4R3me). Unlike other members of the JmjC family, JMJD6 appears to have no lysine demethylase activity . Studies have shown that JMJD6 colocalizes with BRD4 at a subset of enhancers to demethylate H3R2me2 repressor marks. It has also been reported to demethylate non-histone substrates, such as estrogen receptor (ERα), heat shock protein 70 (HSP70), RNA helicase A, and the TRAF6 E3 ubiquitin ligase following activation of toll-like receptors.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of JMJD6 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Histone Lysine Methylation Pathway

Alternative Name :KIAA0585; PTDSR; Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; Histone arginine demethylase JMJD6; JmjC domain-containing protein 6; Jumonji domain-containing protein 6; Lysyl-hydroxylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Phosphatidylserine receptor; Protein PTDSR

Immunogen :Purified recombinant human JMJD6(N-terminus) fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of JMJD6 expression in Hela (A), HL60 (B) whole cell lysates.