Product Name :NRAS Rabbit monoclonal antibody
Reactivity :Human, Mouse, Rat, Hamster
Application_all :WB (1/500 - 1/1000), IP (1/10 - 1/50)
Background :The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms . Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway . GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.
Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Purification&Purity :The antibody was purified by immunogen affinity chromatography.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of NRAS protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Actin Dynamics,Tumor Angiogenesis,ESC Pluripotency and Differentiation Signaling Pathway,ErbB HER Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,Jak Stat ：IL-6 Receptor Signaling,Inhibition of Apoptosis,Insulin &Glucose Signaling,NF-kB Signaling,SAPK JNK Signaling Cascades,T cell receptor signaling,TGFβ Smad signaling,Warburg Effect
Alternative Name :HRAS1; GTPase NRas; Transforming protein N-Ras
Immunogen :A synthetic peptide of human Ras
Modification :Unmodification
