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LC3-I/II Rabbit monoclonal antibody

货号: MB66444

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66444

Product Name :LC3-I/II Rabbit monoclonal antibody

Swiss-Prot :Q9H492

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP

Application_all :WB (1/500 - 1/1000), IP (1/10 - 1/50)

Background :Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer. Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy . Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy . Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles. The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy .

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of LC3-I/II protein.

BiowMW :~ 14 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling

Alternative Name :Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha

Immunogen :A synthetic peptide of human MAP1LC3A

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of LC3-I/II expression in A549 (A), MCF7 (B), 293T (C), C2C12 (D), rat brain (E) whole cell lysates.

miR-210 Regulates Autophagy Through the AMPK/mTOR Signaling Pathway, Reduces Neuronal Cell Death and Inflammatory Responses, and Enhances Functional Recovery Following Cerebral Hemorrhage in Mice

JournalNEUROCHEMICAL RESEARCH

ApplicationWB

IF3.8

SpeciesMouse

PMID40471451