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BCR Rabbit monoclonal antibody

货号: MB66423

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66423

Product Name :BCR Rabbit monoclonal antibody

Swiss-Prot :P11274

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC, IP

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/100), IP (1/10 - 1/50)

Background :The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain, and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase activating function for the small GTP-binding protein Rac. The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Research studies have shown that the Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML). Tyr177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl. Phosphorylated Tyr177 provides a docking site for Gab2 and GRB2.

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of BCR protein.

BiowMW :~ 160 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,PI3K AKT signaling

Alternative Name :BCR1; D22S11; Breakpoint cluster region protein; Renal carcinoma antigen NY-REN-26

Immunogen :A synthetic peptide of human Bcr

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of BCR expression in A549 (A), HL60 (B), U2OS (C), C6 (D) whole cell lysates.

Immunohistochemical analysis of BCR staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.1). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.