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APC Rabbit monoclonal antibody

货号: MB66421

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66421

Product Name :APC Rabbit monoclonal antibody

Swiss-Prot :P25054

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC, IP

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background :The Adenomatous Polyposis Coli (APC) tumor suppressor gene is mutated in most familial and sporadic colorectal cancers and encodes a large cytoplasmic protein that is implicated in cell migration, cell adhesion, and proliferation. APC binds directly to microtubules and lack of APC leads to defective mitotic spindles and aneuploidy due to missegregation of chromosomes. APC is well characterized as a scaffolding protein, binds to β-catenin, and is involved in the regulation of its intracellular concentration. In the absence of a Wnt signal, GSK-3β phosphorylates all three members of the APC-β-catenin-axin complex and this phosphorylation of β-catenin creates a recognition site for ubiquitin, the signal for proteasome-mediated degradation. In the presence of a Wnt signal, dishevelled inactivates GSK-3β and β-catenin coordinates gene transcription of proteins important for the control of cell cycle progression and proliferation, such as cyclin D1 and c-Myc .

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of APC protein.

BiowMW :~ 160 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Adherens Junction signaling,ESC Pluripotency and Differentiation Signaling Pathway,Regulation of Microtubule Dynamics,Wntβ catenin signaling

Alternative Name :DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5

Immunogen :A synthetic peptide of human APC

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of APC expression in Hela (A), A549 (B), HL60 (C), U2OS (D) whole cell lysates.