Product Name :JNK1/2/3 (Phospho-T183/Y185) Rabbit monoclonal antibody
Application_all :WB (1/500 - 1/1000), IP (1/10 - 1/50)
Background :The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase . Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member. There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors.
Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Purification&Purity :The antibody was purified by immunogen affinity chromatography.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of JNK1/2/3 (pT183/Y185) protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Apoptosis,Death Receptor Signaling,ErbB HER Signaling,Inhibition of Apoptosis,Insulin &Glucose Signaling,Mitochondrial Control of Apoptosis,NF-kB Signaling,SAPK JNK Signaling Cascades,T cell receptor signaling,TGFβ Smad signaling,Toll-like Receptor signaling
Alternative Name :JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1
Immunogen :A synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human JNK1
Modification :Phosphorylation
