Western blot analysis of Osteopontin expression in Hela (A), CHOK1 (B), C6 (C), Jurkat (D) whole cell lysates.
Immunohistochemical analysis of Osteopontin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.64). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
