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p53 Rabbit monoclonal antibody

货号: MB66373

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66373

Product Name :p53 Rabbit monoclonal antibody

Swiss-Prot :P04637

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background :The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 . MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation . p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage . Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro . Phosphorylation of p53 at Ser392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 . p53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε both in vitro and in vivo . Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis . Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response. Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding . Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response .

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of p53 protein.

BiowMW :~ 53 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :AMPK Signaling Pathway,Regulation of Apoptosis,ErbB HER Signaling,PI3K AKT signaling.

Alternative Name :P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Immunogen :Recombinant protein of human p53

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of p53 expression in Jurkat (A) whole cell lysates.