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CREB (Phospho-S133) Rabbit monoclonal antibody

货号: MB66332

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66332

Product Name :CREB (Phospho-S133) Rabbit monoclonal antibody

Swiss-Prot :P16220

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC, IP

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/100), IP (1/10 - 1/50)

Background :CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2.

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of CREB (pS133) protein.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling，MAPK Erk in Growth and Differentiation Pathway，Inhibition of Apoptosis，Dopamine Signaling in Parkinson\'s Disease Plathway，Signaling Pathways Activating p38 MAP Kinase，T cell receptor signaling

Alternative Name :Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1

Immunogen :A synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot analysis of CREB (pS133) expression in C6 UV treated (A) whole cell lysates.

Immunohistochemical analysis of CREB (pS133) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.79). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.