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Smooth Muscle Actin Rabbit monoclonal antibody

货号: MB66313

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66313

Product Name :Smooth Muscle Actin Rabbit monoclonal antibody

Swiss-Prot :P62736

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/100)

Background :Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells.

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Smooth Muscle Actin protein.

BiowMW :~ 42 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Death Receptor Signaling

Alternative Name :ACTSA; ACTVS; Actin aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

Immunogen :A synthetic peptide of human alpha smooth muscle Actin

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Smooth Muscle Actin expression in rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.

Immunohistochemical analysis of Smooth Muscle Actin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.53). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.