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ACAT1 Rabbit monoclonal antibody

货号: MB66308

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB66308

Product Name :ACAT1 Rabbit monoclonal antibody

Swiss-Prot :P24752

Host :Rabbit

Reactivity :Human

Applications :WB, IP

Application_all :WB (1/500 - 1/1000), IP (1/10 - 1/50)

Background :Mitochondrial acetyl-coenzyme A (CoA) acetyltransferase 1 (ACAT1) plays a pivotal role in ketogenesis and branched chain amino acid metabolism. Research studies have demonstrated that ACAT1 also plays a key role in carbohydrate metabolism of tumor cells by directly acetylating and inhibiting the activity of the pyruvate dehydrogenase complex (PDH) and PDH phosphatase, which leads to decreased carbon flux through PDH and increased glycolysis. Mechanistically, it has been shown that numerous oncogenic tyrosine kinases directly phosphorylate ACAT1 at Y407, which promotes tetramerization and stabilization of the active enzyme in order to drive glycolysis and tumor growth.

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of ACAT1 protein.

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ACAT; MAT; Acetyl-CoA acetyltransferase mitochondrial; Acetoacetyl-CoA thiolase; T2

Immunogen :A synthetic peptide of human ACAT1

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of ACAT1 expression in K562 (A), Hela (B) whole cell lysates.

免疫沉淀（IP）