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SMAD3 (Phospho-S423/425) Rabbit monoclonal antibody

货号: MB66299

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66299

Product Name :SMAD3 (Phospho-S423/425) Rabbit monoclonal antibody

Swiss-Prot :P84022

Host :Rabbit

Reactivity :Human, Rat, Hamster

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of SMAD3 (pS423/425) protein.

BiowMW :~ 52 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,ESC Pluripotency and Differentiation Signaling Pathway,TGFβ Smad signaling

Alternative Name :MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Immunogen :A synthetic phosphopeptide corresponding to residues surrounding Ser423/Ser425 of human Smad3

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot analysis of SMAD3 (pS423/425) expression in Jurkat (A), C6 (B), CHOK1 (C), Hela (D) whole cell lysates.