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Caspase 3 Rabbit monoclonal antibody

货号: MB66282

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66282

Product Name :Caspase 3 Rabbit monoclonal antibody

Swiss-Prot :P42574

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IP

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/100), IP (1/10 - 1/50)

Background :Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position.

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Caspase 3 protein.

BiowMW :~ 31 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer\'s Disease, Regulation of Apoptosis, Death Receptor Signaling, ErbB HER Signaling, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

Alternative Name :CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Immunogen :A synthetic peptide of human Caspase 3

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Caspase 3 expression in K562 (A), Hela (B) whole cell lysates.

Immunohistochemical analysis of Caspase 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.113). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.