Product Name :PI3K p85 alpha Rabbit monoclonal antibody
Reactivity :Human, Rat, Hamster
Applications :WB, IF/ICC, IP
Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)
Background :Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival. PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β. In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins.
Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Purification&Purity :The antibody was purified by immunogen affinity chromatography.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of PI3K p85 alpha protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :AMPK Signaling Pathway, Regulation of Actin Dynamics, Adherens Junction signaling, Tumor Angiogenesis, Regulation of Apoptosis, ESC Pluripotency and Differentiation Signaling Pathway, ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway, Jak Stat ：IL-6 Receptor Signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Regulation of Microtubule Dynamics, Mitochondrial Control of Apoptosis, NF-kB Signaling, PI3K AKT signaling, Protein Kinase C Signaling, SAPK JNK Signaling Cascades, T cell receptor signaling,TGFβ Smad signaling, Translational Contral elF4 and p70 S6 Kinase, Warburg Effect, MTOR signaling
Alternative Name :GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
Immunogen :A synthetic peptide of human PI 3 Kinase p85 alpha
Modification :Unmodification
