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MLH1 Rabbit monoclonal antibody

货号: MB66267

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66267

Product Name :MLH1 Rabbit monoclonal antibody

Swiss-Prot :P40692

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background :Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligase. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). MLH1 also plays a role in meiotic recombination.

Product :Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of MLH1 protein.

BiowMW :~ 83 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1

Immunogen :A synthetic peptide of human MLH1

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of MLH1 expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.

免疫沉淀（IP）