Product Name :Perforin 1 monoclonal antibody
Application_all :IHC (1/100 - 1/300)
Background :Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells . Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).
Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells . Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.
Product :Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of Perforin 1 protein.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :PFP; Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; PFP
Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within human Perforin 1. The exact sequence is proprietary.
Modification :Unmodification
