Product Name :Actin Sarcomeric Muscle monoclonal antibody
Swiss-Prot :P68133; P68032
Application_all :WB (1/500 - 1/1000), IHC (1/100 - 1/300)
Background :Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility . While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids . For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells . Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin . The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments . Research studies have shown that actin is hyperphosphorylated in primary breast tumors . Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis
Product :Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of Actin Sarcomeric Muscle protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Death Receptor Signaling,
Alternative Name :ACTA; Actin alpha skeletal muscle; Alpha-actin-1; ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin
Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within human Actin Sarcomeric Muscle. The exact sequence is proprietary.
Modification :Unmodification
