Product Name :Estrogen Receptor alpha monoclonal antibody
Application_all :IHC (1/100 - 1/300)
Background :Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains . Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity . Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 . Ser167 may be phosphorylated by p90RSK and Akt . According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients .
Product :Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of Estrogen Receptor alpha protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :MAPK Erk in Growth and Differentiation Pathway,
Alternative Name :ESR; NR3A1; Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within human Estrogen Receptor alpha. The exact sequence is proprietary.
Modification :Unmodification
