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STAT3 monoclonal antibody

货号: MB65978
价格 1350
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :MB65978
Product Name :STAT3 monoclonal antibody
Swiss-Prot :P40763
Host :Mouse
Reactivity :Human, Mouse, Rat
Applications :WB, IHC
Application_all :WB (1/1000 - 1/2000), IHC (1/100 - 1/200)
Background :Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3b appears to be activated by both while Stat3a is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of STAT3 protein.
BiowMW :~ 90 kDa
Note :For research use only, not for use in diagnostic procedure.
Pathway :Immune Cell Signaling,Jak Stat :IL-6 Receptor Signaling,
Alternative Name :APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Immunogen :Recombinant protein corresponding to human STAT3.
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of STAT3 expression in Hela (A), mouse heart (B), rat heart (C) whole cell lysates.
Immunohistochemical analysis of STAT3 staining in human colon cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com