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Phosphotyrosine monoclonal antibody

货号: MB65972

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB65972

Product Name :Phosphotyrosine monoclonal antibody

Host :Mouse

Reactivity :All

Applications :WB, IHC

Application_all :WB (1/1000 - 1/2000), IHC (1/50 - 1/200)

Background :Tyrosine phosphorylation plays a key role in cellular signaling. Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals. Antibodies specific for phospho-tyrosine have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note :For research use only, not for use in diagnostic procedure.

Immunogen :Purified protein corresponding to Phosphotyrosine.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Phosphotyrosine expression in Jurkat (A), 293T (B) whole cell lysates.

Immunohistochemical analysis of Phosphotyrosine staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.