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PCNA monoclonal antibody

货号: MB65969

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB65969

Product Name :PCNA monoclonal antibody

Swiss-Prot :P12004

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/2000 - 1/5000), IHC (1/100 - 1/200)

Background :Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of PCNA protein.

BiowMW :~ 30 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Proliferating cell nuclear antigen; PCNA; Cyclin

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence of human PCNA. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of PCNA expression in Hela (A), 293T (B), NIH3T3 (C), rat brain (D) whole cell lysates.

Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.