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HSP27 monoclonal antibody

货号: MB65955

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB65955

Product Name :HSP27 monoclonal antibody

Swiss-Prot :P04792

Host :Mouse

Reactivity :Human

Applications :WB, IHC

Application_all :WB (1/1000 - 1/2000), IHC (1/100 - 1/200)

Background :Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway. Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers. It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of HSP27 protein.

BiowMW :~ 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Inhibition of Apoptosis,Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Immunogen :Recombinant protein corresponding to human HSP27.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of HSP27 expression in Hela (A) whole cell lysates.

Immunohistochemical analysis of HSP27 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.