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HIF1 beta monoclonal antibody

货号: MB65936

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB65936

Product Name :HIF1 beta monoclonal antibody

Swiss-Prot :P53762

Host :Mouse

Reactivity :Mouse

Applications :WB, IHC

Application_all :WB (1/1000 - 1/2000), IHC (1/100 - 1/200)

Background :Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens (By similarity). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (PubMed:26245371, PubMed:27782878). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of HIF1 beta protein.

BiowMW :~ 88 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BHLHE2; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta

Immunogen :Recombinant protein corresponding to mouse HIF1 beta.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of HIF1 beta expression in mouse brain (A) whole cell lysates.

Immunohistochemical analysis of HIF1 beta staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.