Product Name :Fibronectin monoclonal antibody
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB (1/1000 - 1/2000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)
Background :Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, as well as cell surface integrins to carry on its functions. Fibronectin has been implicated in many essential biological processes including tissue repair, fibrosis, and tumor development. There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the blood stream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly. Fibroblast, endothelial cells, as well as many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets.
Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of Fibronectin protein.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :FN; Fibronectin; FN; Cold-insoluble globulin; CIG
Immunogen :KLH-conjugated synthetic peptide encompassing a sequence of human Fibronectin. The exact sequence is proprietary.
Modification :Unmodification
