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Cleaved PARP1 monoclonal antibody

货号: MB65904

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB65904

Product Name :Cleaved PARP1 monoclonal antibody

Swiss-Prot :P09874

Host :Mouse

Reactivity :Human

Applications :WB, IHC

Application_all :WB (1/2000 - 1/5000), IHC (1/200 - 1/500)

Background :PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Cleaved PARP1 protein.

BiowMW :~ 89 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Death Receptor Signaling,NF-kB Signaling,

Alternative Name :ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence of human Cleaved PARP1. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Cleaved

Western blot analysis of Cleaved PARP1 expression in Jurkat (A) whole cell lysates.

Immunohistochemical analysis of Cleaved PARP1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.