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Beta-catenin monoclonal antibody

货号: MB65887
价格 1350
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :MB65887
Product Name :Beta-catenin monoclonal antibody
Swiss-Prot :P35222
Host :Mouse
Reactivity :Human, Mouse, Rat
Applications :WB, IHC
Application_all :WB (1/1000 - 1/2000), IHC (1/200 - 1/500)
Background :β-catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.
Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of Beta-catenin protein.
BiowMW :~ 92 kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :CTNNB; Catenin beta-1; Beta-catenin
Immunogen :Recombinant protein corresponding to human Beta-catenin.
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of Beta-catenin expression in Hela (A), 293T (B), mouse liver (C), rat liver (D) whole cell lysates.
Immunohistochemical analysis of Beta-catenin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com