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Acetyl Lysine monoclonal antibody

货号: MB65867

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB65867

Product Name :Acetyl Lysine monoclonal antibody

Host :Mouse

Reactivity :All

Applications :WB, IHC

Application_all :WB (1/1000 - 1/2000), IHC (1/200 - 1/500)

Background :Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis. Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport. The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases, and HDACs have become promising targets for anti-cancer drugs currently in development.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Acetyl Lysine protein.

Note :For research use only, not for use in diagnostic procedure.

Immunogen :Purified protein corresponding to Acetyl Lysine.

Conjugate :Unconjugated

Modification :Acetylated

Western blot analysis of Acetyl Lysine expression in mouse brain (A), Hela TSA-treated (B) whole cell lysates.

Immunohistochemical analysis of Acetyl Lysine staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.