Product Name :AKT1 mouse monoclonal antibody (KU)
Reactivity :Human, Dog, Rat, Monkey, Mouse
Applications :FC, IF, IHC, WB
Application_all :WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Background :The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Immunogen :Full length human recombinant protein of human AKT1(NP_005154) produced in HEK293T cell.
Modification :Unmodification
