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CLDN3 mouse monoclonal antibody (KU)

货号: MB4036

价格：￥ ——

货期：现货（1-2个工作日）

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No. :MB4036

Product Name :CLDN3 mouse monoclonal antibody (KU)

Swiss-Prot :O15551

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :IHC, WB

Application_all :WB 1:2000, IHC 1:150

Background :Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). Claudin-3 is the product of an intronless gene (CLDN3) whose expression is markedly dysregulated in distinct cancer subtypes, including ovarian and breast carcinomas (7-9). Somewhat paradoxically, reduced claudin-3 expression is also a hallmark of a subset of breast tumors known as the claudin-low subtype, in which reduced claudin-3 expression was shown to correlate with a phenotype characteristic of cells undergoing epithelial-mesenchymal transition (10).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG2b

BiowMW :~ 20 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Claudin-3; Clostridium perfringens enterotoxin receptor 2 (CPE-R 2; CPE-receptor 2); Cldn3; Cpetr2; 10090 NCBI; Mus musculus (Mouse)

Immunogen :Full length human recombinant protein of human CLDN3 (NP_001297) produced in HEK293T cell.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of CLDN3 mouse monoclonal antibody at 1:500 dilution Lane1:The Liver tissue lysate of Mouse(30ug) Lane:The Lung tissue lysate of Mouse(30ug)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CLDN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, )