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PPM1D mouse monoclonal antibody (KU)

货号: MB4031

价格：￥ ——

货期：现货（1-2个工作日）

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No. :MB4031

Product Name :PPM1D mouse monoclonal antibody (KU)

Swiss-Prot :O15297

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB 1:500~2000

Background :The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG1

BiowMW :~ 65 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein phosphatase 1D; EC:3.1.3.16 ; Protein phosphatase 2C isoform delta (PP2C-delta); Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1; PPM1D; WIP1; 9606 NCBI; Homo sapiens (Human)

Immunogen :Human recombinant protein fragment corresponding to amino acids 1-375 of human PPM1D (NP_003611) produced in E.coli.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PPM1D mouse monoclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(30ug) Lane2:Hela whole cell lysate(30ug)