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PLOD2 mouse monoclonal antibody (KU)

货号: MB4013

价格：￥ ——

货期：现货（1-2个工作日）

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No. :MB4013

Product Name :PLOD2 mouse monoclonal antibody (KU)

Swiss-Prot :O00469

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :IHC, WB

Application_all :WB 1:2000, IHC 1:150

Background :The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG2a

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2; EC:1.14.11.4 ; Lysyl hydroxylase 2 (LH2); PLOD2

Immunogen :Human recombinant protein fragment corresponding to amino acids 359-640 of human PLOD2 (NP_891988) produced in E.coli.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PLOD2 mouse monoclonal antibody at 1:500 dilution. Lane1:U87MG whole cell lysate(30ug) Lane2:PC12 whole cell lysate(30ug) Lane3:A549 whole cell lysate(30ug)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PLOD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)