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PIK3C2A mouse monoclonal antibody (KU)

货号: MB4011

价格：￥ ——

货期：现货（1-2个工作日）

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No. :MB4011

Product Name :PIK3C2A mouse monoclonal antibody (KU)

Swiss-Prot :O00443

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :IHC, WB

Application_all :WB 1:2000, IHC 1:150

Background :Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities. PI3K Class II α generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate. PI3K Class II α is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles. Research studies have indicated that PI3K Class II α regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network. PI3K Class II α also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin. PI3K Class II α has also been shown to regulate neurosecretory granule exocytosis and vascular smooth muscle contraction. Unlike other PI3K family members, PI3K Class II α is less sensitive to the PI3K inhibitors wortmannin and LY294002.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG1

BiowMW :~ 190 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha; PI3K-C2-alpha; PtdIns-3-kinase C2 subunit alpha; Phosphoinositide 3-kinase-C2-alpha; PIK3C2A

Immunogen :Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) produced in E.coli.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PIK3C2A mouse monoclonal antibody at 1:500 dilution. Lane1:SW620 whole cell lysate(30ug)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)