产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

DNM1L mouse monoclonal antibody (KU)

货号: MB4009

价格：￥ ——

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB4009

Product Name :DNM1L mouse monoclonal antibody (KU)

Swiss-Prot :O00429

Host :Mouse

Reactivity :Human, Dog, Rat, Monkey, Mouse

Applications :IHC, WB

Application_all :WB 1:200~4000, IHC 1:150

Background :Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking. DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells. The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission, and this structure is likely conserved in mammalian cells. The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637. Dephosphorylation at Ser637 by calcineurin reverses this inhibition. In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission. Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG2b

BiowMW :~ 81 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Dynamin-1-like protein; EC:3.6.5.5; Dnm1p/Vps1p-like protein (DVLP); Dynamin family member proline-rich carboxyl-terminal domain less (Dymple); Dynamin-like protein; Dynamin-like protein 4; Dynamin-like protein IV (HdynIV); Dynamin-related protein 1; DNM1L

Immunogen :Full length human recombinant protein of human DNM1L(NP_036192) produced in HEK293T cell.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of DNM1L mouse monoclonal antibody at 1:500 dilution. Lane1:HepG2 whole cell lysate(30ug)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)