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CBX3 (5G10) monoclonal antibody

货号: MB0191

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :MB0191

Product Name :CBX3 (5G10) monoclonal antibody

Swiss-Prot :Q13185

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey

Applications :WB ICC IHC IP

Application_all :WB: 1:1000 IP: 1:50~200 ICC: 1:200 IHC: 1:800

Background :At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5´ UTR, have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of CBX3 and does not cross-react with related proteins.

BiowMW :Predicted band size:22KDa Observed band size:22KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :DNA Methylation,

Alternative Name :Chromobox protein homolog 3; HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein; CBX3; HP1γ

Immunogen :Purified recombinant human CBX3 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of CBX3 in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using CBX3 mouse mAb (1:1000 diluted).

Immunohistochemical analysis of paraffin-embedded Prostate Cancer using CBX3 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-CBX3 mouse mAb (dilution 1:200).

Immunoprecipitation analysis of Hela cell lysates using CBX3 mouse mAb.