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ZAP70 (4B2) monoclonal antibody

货号: MB0187

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB0187

Product Name :ZAP70 (4B2) monoclonal antibody

Swiss-Prot :P43403

Host :Mouse

Reactivity :Human

Applications :WB IP

Application_all :WB: 1:1000 IP: 1:50~200

Background :The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes . The TCR subunits include the Ti a and b chains, the CD3 g , d and e chains and a z -containing homodimer or heterodimer . The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains . This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 . ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes . Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 .

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of ZAP70 and does not cross-react with related proteins.

BiowMW :Predicted band size:70KDa Observed band size:70KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,T cell receptor signaling,

Alternative Name :Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK

Immunogen :Purified recombinant human ZAP70 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of ZAP-70 in CEM and Jurkat cell lysates,negative in the Ramos cell lysates using ZAP-70 mouse mAb (1:1000 diluted).

Immunoprecipitation analysis of Jurkat cell lysates (ZAP-70 positive expression cell line)and Ramos cell lysates(ZAP-70 negative expression cell line) using ZAP-70 mouse mAb.