产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Histone H2A.X (phospho-S139) monoclonal antibody

货号: MB0175

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB0175

Product Name :Histone H2A.X (phospho-S139) monoclonal antibody

Swiss-Prot :P16104

Host :Mouse

Reactivity :Human,Mouse

Applications :WB IF

Application_all :WB: 1:1000~2000 IF: 1:200~500

Background :H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139, and does not cross-react with related proteins.

BiowMW :Predicted band size:15KDa Observed band size:15KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Histone Lysine Methylation Pathway,

Alternative Name :Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; γ-H2AX; γH2AX; ganunaH2AX; γ-H2A.X; γH2A.X; ganunaH2A.X

Immunogen :Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).

Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper,untreated or Hydroxyurea-treated) and Hela(lower,untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).

Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).

Regulation of iNOS Expression by NF-κB in Human Lens Epithelial Cells Treated With High Levels of Glucose

JournalInvestigative Ophthalmology & Visual Science

ApplicationWB

IF4.799

SpeciesHuman

PMID23812491
Radiosensitization of esophageal cancer cells ECA109 by knockdown of H2AX

JournalThoracic Cancer

ApplicationCO-IP

IF2.524

SpeciesHuman,Mouse

PMID
Inhibition of DNA-PKcs enhances radiosensitivity and increases the levels of ATM and ATR in NSCLC cells exposed to carbon ion irradiation

JournalOncology Letters

ApplicationIF

IF2.311

SpeciesHuman

PMID26722253
Rheb1 deficiency elicits mitochondrial dysfunction and accelerates podocyte senescence through promoting Atp5f1c acetylation

Journal10.1016/j.cellsig.2024.111451

ApplicationIF,WB

IF4.4

SpeciesMouse,Human

PMID39389178