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PCNA monoclonal antibody

货号: MB0156

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0156

Product Name :PCNA monoclonal antibody

Swiss-Prot :P12004

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey,Hamster

Applications :WB IP ICC

Application_all :WB: 1:1000 ICC: 1:50~100 IP: 1:50~100

Background :The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of PCNA and does not cross-react with related proteins

BiowMW :Predicted band size:36KDa Observed band size:36KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,DNA Methylation,

Alternative Name :Proliferating cell nuclear antigen; Cyclin

Immunogen :Recombinant full length Human PCNA.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of PCNA antibody in Hela, 3T3, COS7, C6 and CHO-K1 cell lysates using PCNA antibody (1:1000 diluted) .

Immunocytochemistry staining of HeLa cells using anti-PCNA antibody (dilution 1:100).Fixed in 100% methanol for 2hr at -20℃.

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