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Raf-B monoclonal antibody

货号: MB0117

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0117

Product Name :Raf-B monoclonal antibody

Swiss-Prot :P15056

Host :Mouse

Reactivity :Human,Mouse

Applications :WB

Application_all :WB:1:300~1000

Background :Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Raf-B mAb detects endogenous levels of Raf-B protein.

BiowMW :Predicted band size:86KDa Observed band size:95KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1

Immunogen :Recombinant full length Human Raf-B.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of BRAF in Hela, Ramos, K562, C2C12 & Jurkat cell lysates using BRAF antibody (1:300 diluted) .