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Cox-4(C-terminus) monoclonal antibody

货号: MB0102

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0102

Product Name :Cox-4(C-terminus) monoclonal antibody

Swiss-Prot :P13073

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey,Goat,Hamster

Applications :WB FC ICC IP

Application_all :WB: 1:1000~5000 ICC: 1:100~200 IP: 1:50~200 FC: 1:50~100

Background :Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain.It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane.The complex consists of 13 mitochondrial- and nuclear-encoded subunits.The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities.The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex.This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme.It is located at the 3´ of the NOC4 (neighbor of COX4) gene in a head-to-head orientation,and shares a promoter with it.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of COX4 and does not cross-react with related proteins

BiowMW :Predicted band size:17KDa Observed band size:17KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :AMPK Signaling Pathway,

Alternative Name :Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; COX4I1; COX4

Immunogen :Synthetic peptide, corresponding to the C-terminus of Human Cox-4.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of COX IV antibody in Goat muscle, CHO-k1, COS7, 3T3, Hela, C6 and K562 cell lysates using COX IV antibody (1:5000 diluted) .

Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.

Immunocytochemistry of HeLa cells using anti- COX4(C-terminus) antibody diluted 1:150.

Immunoprecipitation analysis of Hela cell lysates using COX IV antibody.

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