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Pyruvate Dehydrogenase (C-term) monoclonal antibody

货号: MB0082

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0082

Product Name :Pyruvate Dehydrogenase (C-term) monoclonal antibody

Swiss-Prot :P08559

Host :Mouse

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB: 1/500-1/1000 IF: 1/50-1/200

Background :The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product :Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Pyruvate Dehydrogenase (C-term) monoclonal antibody detects endogenous levels of Pyruvate Dehydrogenase protein.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Warburg Effect,Glutamine Metabolism,AMPK Signaling Pathway,

Alternative Name :Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1; PHE1A

Immunogen :Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of pyruvate dehydrogenase (lipoamide) alpha 1 in COS7, Jurkat, C6, 3T3 and Hela lysates using pyruvate dehydrogenase (lipoamide) alpha 1 antibody.