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CHD3 (C-terminus) monoclonal antibody

货号: MB0061

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0061

Product Name :CHD3 (C-terminus) monoclonal antibody

Swiss-Prot :Q12873

Host :Mouse

Reactivity :Human

Applications :WB IHC FC

Application_all :WB: 1:1000 IHC: 1:500 FC: 1:50~100

Background :This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins

BiowMW :Predicted band size:226KDa Observed band size:260KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ATP-Dependent Chromatin Remodeling Complexes,DNA Methylation,

Alternative Name :Chromodomain-helicase-DNA-binding protein 3; CHD-3; Hzfh; Zinc finger helicase; Mi2-alpha; ATP-dependent helicase CHD3

Immunogen :Synthetic peptide, corresponding to the C-terminus of Human CHD3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of CHD3 antibody in Y79&Raji cell lysates using CHD3 antibody (1:1000 diluted) .

IHC of paraffin-embedded human colon using anti- CHD3 (C-terminus) diluted 1/500-1/1000.

Flow Cytometry analysis of K562 cells stained with CHD3 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.