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TFIIB monoclonal antibody

货号: MB0045

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :MB0045

Product Name :TFIIB monoclonal antibody

Swiss-Prot :Q00403

Host :Mouse

Reactivity :Human

Applications :WB IHC ICC

Application_all :WB: 1:1000 ICC: 1:50~200 IHC: 1:500

Background :Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the “TATA” element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.

Product :Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of TFIIB and does not cross-react with related proteins

BiowMW :Predicted band size:35KDa Observed band size:37KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TF2B; TFIIB; S300-II; Transcription initiation factor IIB

Immunogen :Recombinant full length Human TFIIB.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of TFIIB in Jurkat, MCF7&Hela cell lysates using TFIIB antibody (1:1000 diluted) .

IHC of paraffin-embedded human colon using anti- TFIIB diluted 1/500-1/1000.

Immunocytochemistry of HeLa cells using anti- TFIIB antibody diluted 1:50.

Novel β-phenylacrylic acid derivatives exert anti-cancer activity by inducing Src-mediated apoptosis in wild-type KRAS colon cancer

JournalCell Death & Disease

ApplicationWB

IF6.304

SpeciesHuman,Mouse

PMID30158525