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p53 (C-terminus) monoclonal antibody

货号: MB0038

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0038

Product Name :p53 (C-terminus) monoclonal antibody

Swiss-Prot :P04637

Host :Mouse

Reactivity :Human

Applications :WB ELISA IHC

Application_all :WB: 1:500~1:1000 ELISA: 1:5000~1:10000 IHC: 1:100~1:500

Background :p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active (DNA-binding) conformations and is differentially activated through posttranslational modifications including phosphorylation and acetylation.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of p53 (C-terminus) and does not cross-react with related proteins

BiowMW :~ 53 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Hypoxia Signaling,Warburg Effect,Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint,Examples of Crosstalk Between Post-translational Modifications,Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53

Immunogen :KLH conjugated synthetic peptide derived from human p53.

Conjugate :Unconjugated

Modification :Unmodified

Primary: Anti-P53 at 1/1000 dilution

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P53) Monoclonal Antibody, Unconjugated at 1:500 overnight at 4°C.

Twist induces epithelial-mesenchymal transition and cell motility in breast cancer via ITGB1-FAK/ILK signaling axis and its associated downstream network

JournalInternational Journal Of Biochemistry & Cell Biology

ApplicationWB

IF3.144

SpeciesHuman

PMID26693891