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Cdk1/Cdc2 monoclonal antibody

货号: MB0019

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0019

Product Name :Cdk1/Cdc2 monoclonal antibody

Swiss-Prot :P06493

Host :Mouse

Reactivity :Human

Applications :WB ICC

Application_all :WB: 1:1000 ICC: 1:50~200

Background :Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of Cdk1/Cdc2 and does not cross-react with related proteins

BiowMW :Predicted band size:34KDa Observed band size:34KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2; CDC28A; CDKN1; P34CDC2

Immunogen :Recombinant full length Human Cdk1/Cdc2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of CDC2/CDK1 in MCF7&Hela cell lysates using CDC2/CDK1 antibody (1:1000 diluted) .