产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

TRIM28 (C-terminus) monoclonal antibody

货号: MB0014

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB0014

Product Name :TRIM28 (C-terminus) monoclonal antibody

Swiss-Prot :Q13263

Host :Mouse

Reactivity :Human

Applications :WB IHC ICC

Application_all :WB: 1:1000 ICC: 1:200 IHC: 1:500

Background :Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of TRIM28(C-terminus) and does not cross-react with related proteins

BiowMW :Predicted band size:100KDa Observed band size:100KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; KAP1; TF1B; RNF96; TIF1B; TRIM28

Immunogen :Synthetic peptide, corresponding to the C-terminus of Human TRIM28.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of TRIM28 (C-terminus) in 293T&HepG2 cell lysates using TRIM28 (C-terminus) antibody (1:1000 diluted) .

IHC of paraffin-embedded human Spleen using anti- TRIM28 (C-terminus) diluted 1/500-1/1000.

Immunocytochemistry of HeLa cells using anti- TRIM28 (C-terminus) antibody diluted 1:200.

Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells

JournalBmc Cancer

ApplicationIHC,WB

IF2.933

SpeciesHuman

PMID30309319