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NRF-2 polyclonal antibody（KU）

货号: GCP86

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :GCP86

Product Name :NRF-2 polyclonal antibody（KU）

Swiss-Prot :Q16236

Host :Rabbit

Reactivity :Human,Pig

Applications :WB IHC

Application_all :WB: 1:1000~1:2000 IHC: 1:50~1:200

Background :The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation. Small amounts of constitutive nuclear NRF2 maintain cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes. The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NRF-2 polyclonal antibody detects endogenous levels of NRF-2 protein.

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; Nrf-2; HEBP1; Nuclear factor, erythroid derived 2, like 2; NFE2L2; NRF2

Immunogen :Synthetic peptide, corresponding to Human NRF-2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NRF-2 polyclonal antibody at 1:1000 dilution Lane1:The Brain tissue lysate of Pig(40ug) Lane2:The Heart tissue lysate of Pig(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)

Regulation of antioxidant system, lipids and fatty acid β-oxidation contributes to the cardioprotective effect of sodium tanshinone IIA sulphonate in isoproterenol-induced myocardial infarction in rats

JournalAtherosclerosis

ApplicationWB

IF4.255

SpeciesRat

PMID23958267
Nuclear Factor E2-Related Factor-2 Negatively Regulates NLRP3 Inflammasome Activity by Inhibiting Reactive Oxygen Species-Induced NLRP3 Priming

JournalAntioxidants & Redox Signaling

ApplicationWB

IF5.828

SpeciesHuman,Mouse

PMID27308893
3-(2-Oxo-2-phenylethylidene)-2,3,6,7-tetrahydro-1H-pyrazino[2,1-a]isoquinolin-4(11bH)-one (compound 1), a novel potent Nrf2/ARE inducer, protects against DSS-induced colitis via inhibiting NLRP3 inflammasome

JournalBiochemical Pharmacology

ApplicationWB

IF4.825

SpeciesHuman

PMID26588861
Histone deacetylase inhibition activates Nrf2 and protects against osteoarthritis

JournalArthritis Research & Therapy

ApplicationWB

IF4.103

SpeciesMouse

PMID26408027
Protective Effects of Genistein against Mono-(2-ethylhexyl) Phthalate-Induced Oxidative Damage in Prepubertal Sertoli Cells

JournalBiomed Research International

ApplicationWB

IF2.197

SpeciesRat

PMID29259978