产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Rev-Erbα polyclonal antibody（KU）

货号: GCP108

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :GCP108

Product Name :Rev-Erbα polyclonal antibody（KU）

Swiss-Prot :P20393

Host :Rabbit

Reactivity :Human,Pig

Applications :WB

Application_all :WB: 1:5000~1:10000

Background :Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins. Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells. In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm. Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock. Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2. Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Rev-Erbα polyclonal antibody detects endogenous levels of Rev-Erbα protein.

BiowMW :~ 78 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1; EAR-1

Immunogen :Synthetic peptide, corresponding to Human Rev-Erbα.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Rev-Erbα polyclonal antibody at 1:10000 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:The Lung tissue lysate of Pig(40ug) Lane4:HepG2 whole cell lysate(40ug)