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WT1 polyclonal antibody

货号: BS91456

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS91456

Product Name :WT1 polyclonal antibody

Swiss-Prot :P19544

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB IF IHC ELISA

Application_all :WB: 1:500~1:2000 IHC: 1:100~1:500 IF: 1:100~1:500 ELISA: 1:5000~1:10000

Background :Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino-terminus. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :WT1 polyclonal antibody detects endogenous levels of WT1 protein.

BiowMW :~ 48 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :WIT 2; WT 1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms Tumor; Wilms tumor protein; Wilms' tumor gene; Wilms' tumor protein; WIT2; WT; WT1; WT-1; WT1_HUMAN; WT33

Immunogen :KLH conjugated synthetic peptide derived from human WT1: 301-400/449

Conjugate :Unconjugated

Modification :Unmodification

Sample: TM4 Cell (Mouse) Lysate at 40 ug Primary: Anti-WT1 at 1/300 dilution

Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-WT1 at 1/300 dilution

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-WT1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WT1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

Complement factor B in high glucose–induced podocyte injury and diabetic kidney disease

JournalJCI Insight

ApplicationIF

IF8.315

SpeciesMouse

PMID34622800
Rheb1 deficiency elicits mitochondrial dysfunction and accelerates podocyte senescence through promoting Atp5f1c acetylation

Journal10.1016/j.cellsig.2024.111451

ApplicationIF,WB

IF4.4

SpeciesMouse

PMID39389178